Plasmid

Part:BBa_K112143

Designed by: Madhvi Venkatesh   Group: iGEM08_UC_Berkeley   (2008-11-01)


Entry vector with OriV replication origin

Spectinomycin resistant entry vector with mRFP and OriV flanked by attL sites.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 224
    Illegal BamHI site found at 1134
    Illegal XhoI site found at 3174
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 224
    Illegal XbaI site found at 2021
    Illegal NgoMIV site found at 1238
    Illegal NgoMIV site found at 1245
    Illegal NgoMIV site found at 1369
    Illegal NgoMIV site found at 3027
    Illegal AgeI site found at 872
    Illegal AgeI site found at 984
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None